Characterization of CD133+ parenchymal cells in the liver: Histology and culture

Figure 8 Time-dependent alteration of CD133 expression in HuH7 and HuCCT1 cells. Real-time quantitative RT-PCR revealed CD133 expression levels gradually decreased in non-sorted cells after passage. CD133⁺ HuH7 and HuCCT1 cells showed decreased expression of CD133. In contrast, CD133 expression increased in CD133^- cells in both cell lines. Around day 21-28, CD133 expression in three types of cells became similar to the level in both cell lines.