Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Mar 14, 2009; 15(10): 1153-1161
Published online Mar 14, 2009. doi: 10.3748/wjg.15.1153
Table 1 In-vitro effects of antioxidants on pancreatic islets
AuthorsSubstance/DoseSampleStudy designDurationAssessments
Campbell et al[21], 2008Sodium selenite (30 nmol/L)RatIslets were incubated with/without Na2SeO372 hInsulin content ↑ Insulin secretion ↑ Glucose-stimulated insulin secretion1
Kanitkar et al[22], 2008Curcumin (10 μmol/L)RatCryopreservation of islets with/without curcumin Assessments were performed after 24 h culture post-thawingCryopreservedIntact islets ↑ ROS ↓ Insulin Secretion ↑ Expression of Hsp70 and HO-1 ↑
Thomas et al[23], 2007Peptide SS-31 (1 nmol/L)MiceSS-31 was added to all reagents which were used for islet isolationIsolation procedureIslet apoptosis ↓ Islet yield ↑
HumanIslets were incubated in culture medium with without SS-3172 hIslet apoptosis ↓
Hara et al[24], 2007Epigallocatechin-3-gallate (EGCG) (36, 72, 360 μmol/L)RatIslets cultured under normal or H/R condition with/ without EGCG48 hIslet apoptosis ↓ LDH ↓ Insulin secretion ↑
Xiong et al[25], 2006Puerarin (10, 50, 100 μmol/L)RatIslets cultured under normal or H2O2 stress conditions with/without Puerarin24 hIslet apoptosis ↓, Islet viability ↑ CAT & SOD activity ↑, ROS ↓ Insulin secretion ↑
Marzorati et al[26], 2006Glutathione (1, 5, 10 mmol/L) Vitamin E (2 × 10-5 mmol/L) Ascorbic acid (0.3 mmol/L)HumanAntioxidant agents were added to culture medium individually48 hGlutathione : CCL2/MCP-1 release ↓ Insulin secretion1 Vitamin E: CCL2/MCP-1 release1 Ascorbic acid: CCL2/MCP-1 release1
Amoli et al[27], 2006Curcumin (10, 20 μmol/L)RatCurcumin was added to culture medium18 h + 24 hMCP-1 release ↓
Rao et al[28], 2005MCI – 186RatIslets were treated with H2O2 in the presence or absence of MCI-18618 hCell death ↓
Avila et al[29], 2005L-glutamine (5 mmol/L)HumanPancreas were manually perfused through the main pancreatic duct with either the standard HBSS or with HBSS + L-glutamine0 → 24 hIslet yield ↑ Lipid peroxidation (MDA) ↓ Glutathione (GSH) ↑ Viability1 Insulin secretion1
Brandhorst et al[30], 2005Free L-glutamine (2.5 & 5 mmol/L/L) Stable L-glutamine [ NALG] (2.5 & 5 mmol/L)PigIslets pretreated with free L-glutamine or NALG for 24 h and then stressed with H2O2, ETA or cytokine mix24 hViability: L-glutamine ↑ NALG1
Giovagnoli et al[31], 2005Encapsulated enzymes (100 mg/35 mL)NPCCsNPCCs were co-cultured with/without entrapped enzymes9 dViability ↑, Insulin secretion ↑ Insulin/DNA ratio ↑ mRNA expression of insulin and Glut-2 ↑
Bottino et al[32], 2004MnTDE (34 μmol/L)HumanMnTDE was added as a supplement to culture or isolation medium60 hIslet yield ↑, Insulin secretion ↑ NF-κB DNA-binding ↓ IL6 & MCP-1 ↓, IL81 PARP activation ↑
Arata et al[33], 2004Ascorbic Acid-2 Glucoside (AA2G) (100 μg/mL)HumanCryopreservation of islets with UW solution or AA2G + UW solutionCryopreservedViability ↑ Insulin secretion ↑ Insulin gene expression ↑
Luca et al[34], 2003Free vitamin D3 (2 μmol/L) Encapsulated vitamin D3 (20 μmol/L)RatIslets were treated with/ without vitamin D39 dInsulin secretion ↑
Hardikar et al[35], 2001Taurine (0.3 & 3 mmol/L)RatCryopreservation of islets with/without taurineCryopreservedViability ↑ Lipid peroxidation ↓
Luca et al[36], 2000Vitamin D3 Vitamin ENPCCsNPCC cells were treated with/without VitD3 & VitE during their maturation and differentiation process16 dLarge & intact islets ↑ Insulin secretion ↑
Tajiri et al[37], 1999Vitamin E (50 μg/mL)RatIslets were co-cultured with/ without vitamin E1 dInsulin secretion ↑
Shewade et al[38], 1999RiboflavinRatInclusion of riboflavin in the cryopreserved mediumCryopreservedViability ↑ Lipid peroxidation ↓ GSH ↑, Insulin secretion ↑
Jindal et al[39], 1996Zinc (20 μmol/L)RatPreservation of islets in UW and Hanks solution with/without Zinc1, 3, 6 dViability1