Rapid Communication
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 7, 2006; 12(9): 1452-1457
Published online Mar 7, 2006. doi: 10.3748/wjg.v12.i9.1452
Figure 3
Figure 3 Effects of tunicamycin on expression of S-protein. HEK293 cells were infected with Ad-S/MS virus for 48h in the presence or absence of tunicamycin (7μg/mL). The media were mixed with Ni-NTA-agarose beads and bound protein was eluted with imidazole elution buffer. The pelleted cells were lysed. After centrifugation, the supernatant with eluted protein from the media was separated on SDS-PAGE gels (7%, v/v). Separated proteins were detected by Western blotting using anti-Myc antibody.