Rapid Communication
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 7, 2006; 12(9): 1452-1457
Published online Mar 7, 2006. doi: 10.3748/wjg.v12.i9.1452
Figure 2
Figure 2 Expression and purification of fused S-protein soluble. S-protein fused with Myc/His was expressed in HEK293 cells mediated by adenovirus and purified on Ni-NTA-agarose column and eluted with imidazole at 6 fractions. The eluted proteins were separated by 7% SDS-PAGE gels and identified by both silver staining (A) for elutions 4, 5 and Western-blotting using anti-Myc antibody (B) for elutions 1-5.