Liu CM, Tung TH, Chou P, Chen VTK, Hsu CT, Chien WS, Lin YT, Lu HF, Shih HC, Liu JH. Clinical correlation of gallstone disease in a Chinese population in Taiwan: Experience at Cheng Hsin General Hospital. World J Gastroenterol 2006; 12(8): 1281-1286 [PMID: 16534886 DOI: 10.3748/wjg.v12.i8.1281]
Corresponding Author of This Article
Dr Jorn-Hon Liu, Cheng Hsin General Hospital, Shih-Pai, 112, Taipei, Taiwan, China. email@example.com
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Received: May 18, 2005 Revised: July 25, 2005 Accepted: August 26, 2005 Published online: February 28, 2006
AIM: To explore the prevalence of gallstone disease (GSD) in Taiwan and condition-associated factors related to it.
METHODS: We studied a total of 2386 healthy adults (1235 males and 1151 females) voluntarily admitted to Cheng Hsin General Hospital for a paid physical check-up between January 2002 and December 2002. Blood samples and ultrasound sonography results were collected.
RESULTS: The overall prevalence of GSD among this study-population was 5.3%, including 1.7% (n = 40) having a single stone, 2.3% (n = 55) having multiple stones, and 1.3% (n = 31) having cholecystectomy. The prevalence revealed a statistically significant increase with increasing age (P < 0.0001). Females exhibited a greater prevalence of multiple stones than did males (3.0% vs 1.7%, P = 0.04). Using multiple logistic regression analysis, the following appeared to be significantly related to the prevalence of GSD: older age (40-49 years vs <40 years, OR = 1.63 [95% CI: 0.76-3.48], 50-59 years vs <40 years, OR = 4.93 [95% CI: 2.43-9.99], 60-69 years vs <40 years, OR = 6.82 [95% CI: 3.19-14.60], ≥70 years vs <40 years, OR = 10.65 [95% CI: 4.78-23.73]), higher BMI (≥27 kg/m2 vs <24 kg/m2, adjusted OR = 1.74 [95% CI: 1.04-2.88]), and higher FPG (≥ 126 mg/dL vs <110 mg/dL, OR = 1.71, 95%CI: 1.01-2.96).
CONCLUSION: Older age (≥50 years), obesity (BMI ≥27 kg/m2), and type 2 diabetes (FPG ≥126 mg/dL) are associated with the prevalence of GSD.
Citation: Liu CM, Tung TH, Chou P, Chen VTK, Hsu CT, Chien WS, Lin YT, Lu HF, Shih HC, Liu JH. Clinical correlation of gallstone disease in a Chinese population in Taiwan: Experience at Cheng Hsin General Hospital. World J Gastroenterol 2006; 12(8): 1281-1286
Gallstone disease (GSD) is one of the most common diseases in developed countries. Recent studies indicate varying prevalence of GSD with several predisposing factors in different study populations[1-9]. From a medical economic perspective, the direct and indirect costs of treating GSD patients were estimated at $16 billion and account for more than 800 000 hospitalizations yearly in the United States[9,10]. In Taiwan, the prevalence of GSD in the general population was 4.3% in 1989 while another voluntary screening revealed that the prevalence of GSD was 10.7% among healthy subjects in 1995. For this reason, due to westernization of the diet and the environment, GSD is not rare in the Chinese population and has become one of the major health problems in Taiwan. Without an appropriate and effective screening program for symptomatic GSD, the medical treatment of GSD and related complications contributes substantially to health care costs.
From the viewpoint of preventive medicine, it is not only important to be cognizant of the background prevalence of GSD regionally, but also to explore the complete spectrum of demographic and biological markers which may be related to development of GSD. Although most epidemiologic studies of GSD, demographic factors and biochemical markers have used case-control designs and cross-sectional data[1-9], to the best of our knowledge, some uncertainty still exists regarding the prevalence of the disease and the identity of the associated risk factors for the development of GSD. The present study was designed to explore potential associated risk factors and to improve understanding of the overall pathogenesis of GSD. The purpose of this study was to explore the context of associated risk factors for GSD prevalence amongst the general population aged 20 years or more, as determined by the application of a healthy volunteer subjects screening program at Cheng Hsin General Hospital, a fully certified regional hospital and teaching hospital in Taipei, Taiwan.
MATERIALS AND METHODS
Data resource and data collection
This cross-sectional study was conducted with a total of 2386 healthy adults (1235 males and 1151 females) voluntarily admitted to Cheng Hsin General Hospital for a paid physical check-up between January 2002 and December 2002. Blood samples and ultrasound sonography results were collected. Overnight-fasting blood samples were drawn via venipuncture from study participants by clinical nurses. Serum and plasma samples (from whole blood preserved with EDTA and NaF) were kept frozen (-20 °C) until ready for analysis. The study-used definition of type-2 diabetes was from the 1997 ADA criteria. Definitions of the following diseases / conditions were obesity: a body mass index (BMI)≥27 Kg/m2, high systolic blood pressure (SBP) ≥140 mmHg, high diastolic blood pressure (DBP) ≥90 mmHg, hypercholesterolemia (≥200 mg/dL), hypertriglyceridemia (≥200 mg/dL), low HDL (<35 mg/dL), high BUN (≥20 mg/dL), high creatinine (≥1.4 mg/dL), and hyperuricemia (≥7 mg/dL for males or ≥6 mg/dL for females). Serum ALT or AST levels ≥40 U/L were classified as elevated. In addition, information on HBV and anti-HCV were collected using radioimmunoassay kits.
Diagnosis of gallstone disease
In the present study, GSD was diagnosed by a panel of specialists using real-time ultrasound sonography (TOSHIBA nemio SSA-550A, Japan) to examine the abdominal region after fasting for at least 8 h based on the presence of “movable hyper-echoic foci with acoustic shadow. Cases of GSD were classified as follows: single gallbladder stone, multiple gallbladder stones, and cholecystectomy, excluding gallbladder polyps. Cases were identified as any type of GSD study population.
Interobserver reliability in ultrasound sonography
In order to set up a consistent diagnosis of GSD between specialists, the Kappa statistic was used to assess the agreement of inter-observer reliability among study specialists. A pilot study was performed using 100 randomly selected healthy subjects other than the study participants. For inter-observer reliability, the Kappa value for diagnosis of GSD between specialists was 0.79 (95%CI: 0.61-0.95).
Statistical analysis was performed using SAS for Windows, (SAS version 9.0; SAS Institute Inc., Cary, NC, USA). A P-value of < 0.05 was considered statistically significant between two test populations. For univariate analysis, the two-sample, independent t-test method was adopted to assess differences in the mean value of continuous variables between subjects with and without GSD. Crude and adjusted odds ratios (adjustment for gender and age) were estimated and 95% confidence intervals were used. Multiple logistic regression was also performed in order to investigate the independence of risk factors associated with the prevalence of GSD.
The prevalence of each type of GSD amongst study subjects is presented in Table 1. The overall prevalence of GSD was 5.4%, including 1.7% (n = 40) having a single stone, 2.3% (n = 55) having multiple stones, and 1.3% (n = 31) having cholecystectomy. Females (3.0%) had a significantly higher prevalence of multiple stones than males (1.7%) (P = 0.04). There were no statistically significant differences in gender for other types of GSD. In addition, from the Cochran-Armitage trend test, the prevalence of each type of GSD showed an increase with age (P < 0.0001). Subjects aged 50 years and over (96/918 = 10.5%) had a more than 5-fold risk for GSD compared with the subjects aged 50 years and less (30/1468 = 2.0%).
Table 1 Prevalence of each type of gallstone disease among 2386 Chinese subjects.
P-value for χ2-test
P-value for Cochran-Armitage trend test
Figure 1 shows the gender- and age-specific prevalence of all types of GSD. Although there was no gender difference (χ2 = 1.30, P = 0.25) for the overall prevalence of GSD, there was a statistically significant increase with increasing study-subject age by means of the trend test (z = 9.66, P < 0.0001). The prevalence of GSD also showed an interaction effect between gender and age, that is, males proved to have a substantially greater overall prevalence of GSD than females among those aged less than 50 years, whereas there was a lower overall prevalence of GSD among those aged more than 50 years.
Figure 1 Gender- and age-specific prevalence of all types of gallstone disease among 2386 Chinese subjects.
The results of the comparison of a variety of test characteristics and their potential association with the prevalent GSD for study subjects are illustrated in Table 2. Using a two-sample independent t-test, the associated factors that were significantly related to GSD included age (yes [58.63 ± 13.04 years] vs no [46.57 ± 13.22 years]), BMI (yes [24.83 ± 3.64 Kg/m2] vs no [23.50 ± 3.55 Kg/m2]), fasting plasma glucose (FPG) (yes [109.22 ± 33.21 mg/dL] vs no [96.98 ± 25.21mg/dL]), SBP (yes [130.06 ± 20.07 mmHg] vs no [122.55 ± 18.40 mmHg]), and BUN (yes [14.94 ± 6.28 mg/dL] vs no [13.55 ± 5.56 mg/dL]).
Table 2 Comparison of characteristics in subjects with and without gallstone disease.
Any type of gallstone disease
Yes(n=126)Mean ± SD
No(n=2260)Mean ± SD
Total(n=2386)Mean ± SD
P-valuefor t test
58.63 ± 13.04
46.57 ± 13.22
47.21 ± 13.48
24.83 ± 3.64
23.50 ± 3.55
23.57 ± 3.57
109.22 ± 33.21
96.98 ± 25.21
97.63 ± 25.84
130.06 ± 20.07
122.55 ± 18.40
122.94 ± 18.56
81.27 ± 12.22
79.56 ± 12.56
79.65 ± 12.55
216.93 ± 41.06
210.51 ± 37.72
210.87 ± 37.94
143.39 ± 98.63
130.12 ± 108.06
130.86 ± 107.58
57.02 ± 16.55
57.69 ± 15.76
57.65 ± 15.80
14.94 ± 6.28
13.55 ± 5.56
13.63 ± 5.61
1.08 ± 0.35
1.04 ± 0.40
1.04 ± 0.40
Uric acid (mg/dL)
6.74 ± 1.67
6.59 ± 1.72
6.60 ± 1.72
32.79 ± 30.13
27.59 ± 17.59
27.88 ± 18.54
37.84 ± 54.27
30.01 ± 34.49
30.45 ± 35.91
Table 3 presents the crude and adjusted odds ratios for the association between certain relevant associated risk factors and the prevalence of GSD. Compared to individuals without GSD, in addition to older age (40 - 49 years vs <40 years, OR = 1.80 [95% CI: 0.85 - 3.81], 50-59 years vs <40 years, OR = 5.75 [95% CI: 2.92-11.32], 60-69 years vs <40 years, OR = 9.02 [95% CI: 4.45-18.29], ≥70 years vs <40 years, OR=12.76 [95% CI: 6.16-26.43]), subjects featuring GSD revealed a more-pronounced prevalence of: higher BMI (≥27 kg/m2vs <24 kg/m2, adjusted OR = 2.04 [95% CI: 1.28-3.26]), higher FPG (≥126 mg/dL vs <110 mg/dL, OR = 1.95, 95% CI: 1.16-3.30), and higher AST (adjusted OR=1.96, 95%CI: 1.16-3.32) subsequent to adjustment for gender and age.
Table 3 Univariate analysis of associated clinical factors for gallstone disease among 2386 Chinese subjects.
The effect of independent associated risk factors on GSD was examined using a multiple logistic regression model. As depicted in Table 4, subsequent to adjustment for confounding factors, the following appeared to be significantly related to GSD prevalence: age (40-49 years vs <40 years, OR = 1.63 [95% CI: 0.76-3.48], 50-59 years vs <40 years, OR = 4.93 [95%CI: 2.43 - 9.99], 60 - 69 years vs <40 years, OR = 6.82 [95% CI: 3.19 - 14.60], ≥70 years vs <40 years, OR = 10.65 [95% CI: 4.78 - 23.73]), the higher BMI (≥27 kg/m2vs <24 kg/m2, adjusted OR = 1.74 [95% CI: 1.04 - 2.88]), and higher FPG (≥126 mg/dL vs <110 mg/dL, OR = 1.71, 95% CI: 1.01 - 2.96).
Table 4 Multiple logistic regression of associated factors for gallstone disease among 2386 Chinese subjects.
Gallstone disease(yes vs no)
Gender (female vs male)
(40-49 vs <40 yr)
(50-59 vs <40 yr)
(60-69 vs <40 yr)
(≥70 vs <40 yr)
(24-27 vs <24 kg/m2)
(≥27 vs <24 kg/m2)
(110-125 vs <110 mg/dL)
(≥126 vs <110 mg/dL)
High SBP (yes vs no)
High DBP (yes vs no)
Hypercholesterolemia (yes vs no)
Hypertriglyceridemia (yes vs no)
Low HDL (yes vs no)
High BUN (yes vs no)
High creatinine (yes vs no)
Hyperuricemia (yes vs no)
Higher AST (yes vs no)
Higher ALT (yes vs no)
HBV (positive vs negative)
Anti-HCV (positive vs negative)
Prevalence of gallstone disease in the general population
One of the important benefits of early screening for GSD is that ultrasonography can lead to the discovery of an increasing proportion of asymptomatic cases for which one can use therapeutic nonsurgical approaches, such as litholitic bile acids, local solvents, and lithotripsy. However, it appears that only a few published studies have attempted to determine the prevalence and possible etiology of GSD prevalence for the general population of Taiwan[2,8,14], which also face a GSD burden. In the present study, GSD appeared to be fairly common for the test population, affecting an estimated 5.3% of the general population in Taiwan. The prevalence of GSD amongst different test populations appears to vary, differing among different studies conducted in different countries[1,3-6]. In addition to different methods of GSD assessment, this disparity might be due to differences between different population stocks. The prevalence of GSD for our study population (5.3%) was lower than the corresponding figure presented in a previous hospital-based study conducted in the Veterans General Hospital (VGH), Taipei, Taiwan, which was reported to be 10.7%. The apparent lower prevalence rate in our study may have been due to younger age of the participants. Another possible reason for such differences between the results of the VGH study and our results may simply have been related to the fact that although the kappa value for agreement of interobserver reliability seemed good , non-differential misclassification-bias identification could still be occurring and could lead to underestimation of GSD prevalence.
The implications of associated risk factors for gallstone disease
Our results reveal that older age represents a significant risk factor for GSD. Such a finding is consistent with results of other hospital-based and community-based studies conducted elsewhere[2-5,7,8]. GSD is very seldom found in children, and in that age group is mainly associated with haemolytic causes. The long-term exposure to many other risk factors among elder persons may also account for the increased probability of developing GSD. Cholelithiasis is also an acquired disease contributed to by chronic environmental factors plus an aging effect. In addition, it has been suggested that among the elderly, larger amounts of cholesterol are secreted by the liver, and the catabolism of cholesterol to bile acid is decreased[16,17].
Most previous epidemiologic studies have shown that females have a higher prevalence of GSD than males in the Western world. However, the male to female ratio seems to have changed from early reports, which showed figures of 1:4-6 to more recent studies where the ratio is 1:2 or less[3,4,18]. Pregnancy and sex hormones could be involved by altering biliary secretion or gallbladder motility, or both may play an important role in sex-related differences in the prevalence of GSD. Another possible reason might be that estrogen replacement therapy or oral contraceptives are used. Our findings showed that, except for multiple stones, females do not have a significantly higher prevalence of all types of GSD than males. This result is similar to that from other hospital-based or population-based studies conducted in Taiwan[2,7,8].
After adjustment for confounding factors, the present study also suggests that obesity is highly correlated with GSD prevalence. Supersaturated bile is the linkage between obesity and cholesterol GSD. Obesity can raise the saturation of bile by increasing biliary secretion of cholesterol - the latter probably depending on a higher synthesis of cholesterol in obese subjects[7,13,20]. While cholesterol GSD is common in Western countries, pigment GSD is still the principal component in Taiwan. Our results also implied that obesity may be an independent associated risk factor leading to prevalent pigment GSD in the Chinese population.
It has been a matter of controversy whether diabetes mellitus is associated with GSD or not. Consistent with previous studies[4,7], our results showed that diabetes mellitus is highly associated with GSD prevalence. From a clinical perspective, hyperglycemia inhibits bile secretion from the liver and disturbs gallbladder contraction . Supersaturated bile in the gallbladder induces cholelithiasis in diabetic subjects. The association of diabetics with GSD is stronger in subjects with a history of treated diabetes mellitus than in those with a simple history of diabetes and this could be an effect of hyperglycemia on gallbladder motility. Furthermore, diabetes mellitus combined with GSD may induce acute cholecystitis more frequently and have a higher possibility of progression to septicemia.
An increasing prevalence of cholecystolithiasis has been associated with the etiology and severity of chronic liver disease and cirrhosis. Possible explanatory factors include overproduction and over-secretion of bilirubin due to increased extracorpuscular hemolysis, a reduction in the production and transportation of biliary salts and cholesterol secretion, stasis and changes in bile acidification, or even mucous hypersecretion and subtle alterations in gallbladder mucosal function[24,25]. However, although early liver dysfunction such as abnormal ALT, HBV, and HCV infection are endemic in Taiwan[12,26,27], the present study did not reveal liver function associated factors to be related to GSD prevalence. This finding is consistent with other hospital-based studies for the general population in Taiwan. Further epidemiological and etiologic investigations are needed to clarify the pathophysiological mechanisms between early liver damage and pigment GSD among general Chinese populations.
A major limitation of the present study is the potential self-selection bias due to the hospital-based study design, that is, of it not being exactly representative of the whole general population. Second, it is well established that a large proportion of GSD patients remain asymptomatic and they are therefore often ignored for many years. Thus the present study may be representative of the clinical and not of the true prevalence. Thirdly, we do not detail to distinguish between cholesterol stones and pigment stones in this study, some measurement errors and different pathogenicity could occur. Fourthly, we did not consider how many individuals had progressive liver disease and explore the relationship between GSD and liver disease. Finally, our measurements were done only at a single time point and could not be able to be reflect long-term exposure to various demographic or biochemical aspects or factors, factors which might be important influencers of GSD. The solution to such a quandary is to conduct a number of prospective longitudinal analogous studies, the results of which would be expected to complement the cross-sectional findings of this study.
In conclusion, older age, obesity (BMI≥27 kg/m2), and type 2 diabetes (FPG≥126 mg/dL) are associated with GSD prevalence. Further studies are needed not only to elucidate the temporal sequence of events that typically lead to GSD, but also to further explore the pathogenesis of GSD in the general Chinese population.
S- Editor Wang J L- Editor Guo SY E- Editor Liu W
Diehl AK, Schwesinger WH, Holleman DR Jr, Chapman JB, Kurtin WE. Clinical correlates of gallstone composition: distinguishing pigment from cholesterol stones.Am J Gastroenterol. 1995;90:967-972.
Wang CS, Chang TT, Yao WJ, Chou P. Comparison of hepatitis B virus and hepatitis C virus prevalence and risk factors in a community-based study.Am J Trop Med Hyg. 2002;66:389-393.
Liu CM, Tung TH, Liu JH, Chen VT, Lin CH, Hsu CT, Chou P. A community-based epidemiological study of elevated serum alanine aminotransferase levels in Kinmen, Taiwan.World J Gastroenterol. 2005;11:1616-1622.