Study on relationship between expression level and molecular conformations of gene drugs targeting to hepatoma cells in vitro

Figure 1 Primers for amplifying human interleukin 12.

Figure 2 Electrophoresis of P (-)/P₃₅, P (+)/P₄₀ and their bands after restrictive endonuclease enzyme digestion in a 0. 8% aga-rose gel. 1. P (-)/P₃₅ plasmid digested by Kpn I and Nhe I. 2. P (-)/P₃₅ plasmid. 3. Marker (λDNA/Hind III). 4. P (+)/P₄₀ plasmid. 5. of P (+)/P₄₀ plasmid cut by Kpn I and Xho I.

Figure 3 Electrophoresis of recombinant expression plasmid P (+)/IL-12 and the bands after restriction enzyme digestion in a 0. 8% agarose gel (Marker: λDNA/Hind III).

Figure 4 Differently structural features of targeting gene drugs (ASOR-PLL-DNA complexes) at various concentrations of adjuvant under transmission electron microscope. (The bar equals 100 nm, amplified 40000 times).

Figure 5 ELISA results of hIL-12 expressed in cell supernatant 48 hours after targeting gene drugs at various adjuvant concentrations were transfected into HepG2.