hOGG1 Ser326Cys polymorphism modifies the significance of the environmental risk factor for colon cancer

Figure 1 A: The representative PCR-RFLP analysis of hOGG1 Ser326Cys polymorphism. Lane 1, 5, undigested PCR-ampli-fied product observed in homozygous Ser326 carriers; Lane 2, Fsp4HI-digested PCR product from subject with heterozygous hOGG1 genotype; Lane 3, 4, Fsp4HI-digested PCR product from subject with homozygous Cys326 genotype. B: DNA se-quence histogram revealing the indicated sequence changes (underlined). In the 2nd histogram, both the cytosine (blue line) and guanine (black line) are observed in the codon 326 site (marked as N), indicating the heterozygous genotype.