Recombinant Helicobacter pylori catalase

doi:10.3748/wjg.v9.i5.1119

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May 15, 2003 (publication date) through Apr 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 15, 2003; 9(5): 1119-1122
Published online May 15, 2003. doi: 10.3748/wjg.v9.i5.1119

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Figure 1 Agarose gel electrophoresis of PCR products and plasmid pET-CAT digested with restriction enzymes. Lane 1: DNA marker; Lane 2: PCR product; Lane 3: pET-22b (+)/Xho I; Lane 4: pET-CAT/Xho I; Lane 5: pET-CAT/Nco I + Not I.

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Figure 2 SDS-PAGE analysis of catalase recombinant protein expressed in BL21 (DE3). Lane 1: Molecular weight marker (20, 30, 43, 67, 94) × 10³; Lane 2: BL21 (pET-CAT) cells before induction; Lane 3: BL21 (pET-CAT) cells after 3 h induction with IPTG; Lane 4: BL21 (pET-CAT) cells periplasm protein after 3 h induction with IPTG; Lane 5: sonicate supernatant of BL21 (pET-CAT) cells after 3 h induction with IPTG; Lane 6: inclusion body of BL21 (pET-CAT) cells after 3 h induction with IPTG; Lane 7: control strain BL21 (pET) before induction; Lane 8: control strain BL21 (pET) after 3 h induction with IPTG.

Citation: Bai Y, Zhang YL, Jin JF, Wang JD, Zhang ZS, Zhou DY. Recombinant Helicobacter pylori catalase. World J Gastroenterol 2003; 9(5): 1119-1122
URL: https://www.wjgnet.com/1007-9327/full/v9/i5/1119.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i5.1119