A mutation specific polymerase chain reaction for detecting hepatitis B virus genome mutations at nt551

Figure 1 Amplification of Control HBV DNA. Lane 1: DNA Marker; Lane 2: HBV DNA with an A at nt551 amplified by P551A-PPS; Lane 3: HBV DNA with a G at nt551 amplified by P551G-PPS; Lane 4: HBV DNA with a C at nt551 amplified by P551CM-PPS; Lane 5: HBV DNA with a T at nt551 amplified by P551TM-PPS. The amplified DNA fragments are 314 bp long

Figure 2 Establishment of the msPCR for nt551A and nt551G Lane 1: DNA Marker; Lane 2-5: P551A-PPS amplified HBV DNAs of control (nt551A in Lane 2, nt551G in Lane 3, nt551C in Lane 4, nt551T in Lane 5). Lane 6-9: P551G-PPS amplified HBV DNAs of control (nt551A in Lane 6, nt551G in Lane 7, nt551C in Lane 8, nt551T in Lane 9).

Figure 3 Establishment of the msPCR for nt551C and nt551T Lane 1: DNA Marker; Lane 2-5: P551C-PPS amplified HBV DNAs of control (nt551A in Lane 2, nt551G in Lane 3, nt551C in Lane 4, nt551T in Lane 5). Lane 6-9: P551T-PPS amplified HBV DNAs of control (nt551A in Lane 6, nt551G in Lane 7, nt551C in Lane 8, nt551T in Lane 9).

Figure 4 NO. 2, NO.5, NO.8 and NO.57 are compared with the know adr subtype HBV genome. The sequence from nt524 to nt595 code for aa124 to aa147 of HBsAg (The first T in the single EcoRI cleavage site of HBV genome is nt1).