Identification of alkA gene related to virulence of Shigella flexneri 2a by mutational analysis

Figure 1 Location of primers P1, P2, P3 and P4 in PCR prod-ucts of alkA and 028pKm. A: 028pKm (about 1.4 kb); B: alkA (about 1.7 kb).

Figure 2 Construction and chromosomal integration of pXLkd46 plasmid.

Figure 3 Construction of recombinant plasmid pMD028pkm.

Figure 4 Verification of alkA gene deletion of 2457T028D mutant by PCR. 1: alkA PCR product (amplified from 2457T05), 2: 028pKm PCR product (amplified from 2457T028D), 3: 028Kkm PCR product (amplified from pMD028pKm), 4: DL2000 Marker.

Figure 5 Comparison of HeLa cells infected by 2457T, 2457T05, 2457T028D and MC1061.

Figure 6 Hypothesis illustrating the relationship between alkA and damage of DNA methylation.