Expression of mucosal addressin cell adhesion molecule 1 on vessel endothelium of gastric mucosa in patients with nodular gastritis

Figure 1 (A): H pylori-negative gastric mucosa showed little immunoreactivity for mucosal addressin cell adhesion mol-ecule 1 (MAdCAM-1). MAdCAM-1 was expressed on the en-dothelium of numerous vessels within the lamina propria with H pylori infection (B), particularly in association with dense mononuclear infiltration (C). Strong endothelial expres-sion of MAdCAM-1 was localized on high endothelial venule-like vessels adjacent to the lymphoid follicles D.

Figure 2 MAdCAM-1 immunoreactivity on mucosal vascula-ture of antral biopsy specimens from patients with nodular gas-tritis and H pylori-positive and –negative controls. Results were expressed as the percentage of von-Willebrand factor-positive vessels immunoreactive for MAdCAM -1 in serial sections.

Figure 3 In simultaneous viewing of serial sections, integrin β7-expressing cells (A) consisted of CD-4-positive T lymphocytes (B).

Figure 4 Vessels lined with MAdCAM-1-positive endothelium (A) were associated with infiltration of lymphocytes immu-noreactive for integrin β7 (B).

Figure 5 MAdCAM-1 and glyceraldehyde-3-phosphate dehy-drogenase (G3PDH) mRNA transcripts were detected as 387 and 983 base pair-bands with reverse transcriptase-polymerase chain reaction, respectively. Lane N: negative control, lane 1: H pylori-negative control, lane 2: H pylori-positive control, lane 3: patients with nodular gastritis.