Construction of expression systems for flaA and flaB genes of Helicobacter pylori and determination of immunoreactivity and antigenicity of recombinant proteins

Figure 1 Target fragments of flaA and flaB genes amplified from H pylori strain Y06 DNA.

Figure 2 Homologies of nucleotide sequence of cloned H pylori flaA gene with reported sequences. (1): the sequencing result of H pylori strain Y06 flaA gene; (2)-(4): the reported sequences from GenBank (No. NC000915, strain 26695; No. NC_000921, strain J99; No. X60746, strain 898-1). Underlined areas indicate the positions of primer sequences.

Figure 3 Homologies of putative amino acid sequence of H pylori flaA gene with reported sequences. (1): the sequencing result of cloned H pylori strain Y06 flaA gene; (2)-(4): the reported sequences from GenBank (No. NC000915, strain 26695; No. NC_000921, strain J99; No. X60746, strain 898-1).

Figure 4 Homologies of nucleotide sequence of cloned H pylori flaB gene with reported sequences. (1) the sequencing result of H pylori strain Y06 flaB gene; (2)-(5): the reported sequences from GenBank (No. NC000915, strain 26695; No. NC_000921, strain J99; No. L08907, strain 85P; No. AF479024, strain CH-CTX1). Underlined areas indicate the positions of primer sequences.

Figure 5 Homologies of putative amino acid sequences of H pylori flaB gene with reported sequences. (1): the sequencing result of H pylori strain Y06 flaB gene; (2)-(5): the reported sequences from GenBank (No. NC000915, strain 26695; No. NC_000921, strain J99; No. L08907, strain 85P; No. AF479024, strain CH-CTX1).

Figure 6 Expression of rFlaA induced by IPTG at different concentrations.

Figure 7 Expression of rFlaB induced by IPTG at different concentrations.

Figure 8 Western blot result of rabbit antibodies against whole cell of H pylori and rFlaA.

Figure 9 Western blot result of rabbit antibodies against whole cell of H pylori and rFlaB.