Identification and characterization of a novel isoform of hepatopoietin

Figure 1 Amino acid sequence alignment of HPO-205 and HPO (GenBank CI 11559825)

Figure 2 The mRNA expression of HPO-205 was determined by RT-PCR in hepatoma cell lines. a: HepG2 cell line; b: HLE cell line; c: SMMC-7721 cell line; d: Bel-7402 cell line; GAPDH as a control.

Figure 3 HPO-205 protein was detected by Western blot in hepatoma cell lines. a: HepG2 cell line; b: Bel-7402 cell line; c: SMMC-7721 cell line; d: HLE cell line

Figure 4 A: The HPO-205 mRNA expression in transfected Cos7 cells was detected by RT-PCR method. a: DNA marker; b: pcDNAHPO-205 vector; c: pcDNA vector; B: The effect of DNA synthesis of HepG2 cells stimulated by the different expressed protein.

Figure 5 The stably transfected cell lines were confirmed and MAPK activation was examined by Western Blot. A. HPO and HPO-205 protein in transfected Bel-7402 cells; B. MAPK levels in different transfected cell lines.