Basic Research
Copyright ©The Author(s) 2002.
World J Gastroenterol. Apr 15, 2002; 8(2): 318-322
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.318
Figure 1
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Figure 1 Scheme and electrophoresis identification of recombinant pGEM-CYP2C9. A: Scheme of recombinat of pGEM-CYP2C9; B: Electrophoresis identification of recombinant pGEM-CYP2C9; 1: Marker (λ/EcoR I and Hind III); 2: PCR product ofCYP2C9 (1.54 kb); 3: Recombinant of pGEM-CYP2C9 digested by Kpn I and Xho I; 4: pGEM-T vector
Figure 2
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Figure 2 Scheme and electrophoresis identification of recombinant pREP9-CYP2C9. A: Scheme of pREP9-CYP2C9; B: Electrophoresis identification of recombinant pREP9-CYP2C9; 1: Marker (λ/EcoR I and Hind III); 2: PCR product ofCYP2C9 (1.54 kb); 3: Recombinant of pREP9-CYP2C9 digested by Kpn I and Xho I; 4: pREP9 vector
Figure 3
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Figure 3 Representative chromatogram of extractsA Shim-pack CLC-ODS column (15 cm ± 0. 6 cm i.d.) was used. The mobile phase was constituted with phosphoric acid (pH2.6), acetonitrile (6:4/V:V) with the flow rate at 1 mL·min-1. Hydroxytolbutamide was monitored at 230 nm. A: hydroxytolbutamide; B: tolbutamide