H. Pylori
Copyright ©The Author(s) 2002.
World J Gastroenterol. Apr 15, 2002; 8(2): 308-311
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.308
Figure 1
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Figure 1 Ten g•L⁻¹ agarose gel electrophoreses of Mr26000 OMP DNA fragment amplified by PCR from Helicobacter pylori. Lane1: Nucleotide marker; Lane2-4: PCR products; Lane 5: Negative control.
Figure 2
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Figure 2 The identification of recombinant plasmid by restriction enzyme digestion. Lane1: Nucleotide marker; Lane2: pET32a(+)/Hind III; Lane3: pET32a(+)/Hind III, BamH I; Lane4: Recombinant plasmid/Hind III; Lane5: Recombinant plasmid/Hind III, BamH I.
Figure 3
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Figure 3 150 g•L⁻¹ SDS-PAGE analysis of the fusion protein expressed in BL21(DE3). Lane1: Molecular weight marker; 2Lane: BL21 after 4 h induction with IPTG; Lane3-9: BL21/recombinant vector expression after 4 h induction with 0.5, 1, 1.5, 2, 2.5, 3, 4 mmol•L⁻¹ IPTG respectively; Lane10: BL21/pET32a(+) vector expression after 4 h induction with IPTG.