VEGF165 antisense RNA suppresses oncogenic properties of human esophageal squamous cell carcinoma

Figure 1 Diagram of the construction of the vector pCEP-AVEGF₁₆₅.

Figure 2 Identification of recombinant clone by restriction enzyme. 1: Fragment of 640 bp digested with Kpn I/Hind III; 2: DL2000 markers (2000,1000,750,500,250,100 bp); 3: Fragment of 660 bp digested with Kpn I/Sfi I.

Figure 3 Expression of antisense RNA to VEGF165 in EC109 cell. 1: Transfected by pCEP-AVEGF165; 2: Transfected by empty vector; 3: Control group.

Figure 4 Expression of VEGF₁₆₅ in Ec109 cell ( × 40): transfected by pCEP-AVEGF₁₆₅ (A), transfected by empty vector (B), and control group (C).