Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells

Figure 1 Results of PCR amplication with HPV-16 E6 primers. 1:100 bp DNA ladder marker. 2: nuclear matrix associated DNA of EC/CUHK1 cells, with 50 mg/L DNase digestion. 3: nuclear matrix associated DNA of EC/CUHK2 cells, with 50 mg/L DNase digestion. 4: nuclear matrix associated DNA of EC/CUHK2 cells, with 100 mg/L DNase digestion. 5: HPV-16 plasmid DNA.

Figure 2 RT-PCR amplification with HPV-16 E6 primers. 1:100 bp DNA ladder marker. 2: EC/CUHK2 cells. 3: EC/CUHK1 cells.

Figure 3 Immunocytochemical staining of anti-HPV-16 E6 protein in nuclear matrix of EC/CUHK2 cells.