Differential expression of a novel colorectal cancer differentiation-related gene in colorectal cancer

Figure 1 Detection of the exponential range by termination at se-quential PCR cycles. 1-7: Cycles of 20, 22, 24, 25, 26, 28 and 30, respectively. M: Marker (100 bp DNA ladder).

Figure 2 Detection of the exponential range by serial dilutions of cDNA. 1-4: cDNA of volume of 5, 2.5, 1.25, and 0.625 μL, respectively. M: Marker (100 bp DNA ladder).

Figure 3 Relative quantification of SBA2 mRNA in colorectal tissues. 1-4: Moderately, moderately, poorly, and well differentiated CRC, respectively. 5: Healthy colorectal mucosa. M: Marker (100 bp DNA ladder).

Figure 4 Relative quantification of SBA2 mRNA in cell lines of CRC. 1-4: Human CRC cell line CloneA, CX1, CCL187, and LS174T, respectively. M: Marker (100 bp DNA ladder).