Extraction and purification of TGFβ and its effect on the induction of apoptosis of hepatocytes

Figure 1 Ion-exchange chromatography of acid/ethanol extract on a CM Sepharose column.

Figure 2 Gel chromatography of the CM-Sepharose peak 2 and peak 3 fractions on a Superdex-75 column.

Figure 3 Molecular mass analysis of the extracted TGFβ (SDS PAGE). Right Lane: marker proteins.

Figure 4 The granulation tissue formation after 72 hrs subcutaneous injection of TGFβ. HE × 400

Figure 5 Hepatocytes QZG displayed apoptosis after being treated with 8 μg/L TGFβ for 12 hrs. HE × 400