Detection of fucosylated haptoglobin using the 10-7G antibody as a biomarker for evaluating endoscopic remission in ulcerative colitis

Figure 1 Schematic illustration of fucosylated haptoglobin detection using the 10-7G mAb. ELISA: Enzyme-linked immunosorbent assay.

Figure 2 Immunohistochemical study of ulcerative colitis and Crohn’s disease intestinal tissues using the 10-7G mAb. A and B: Lymphocytes infiltrating into inflammatory sites of the mucosal layer (A) and lymph nodules in intestinal tissues (B) of patients with ulcerative colitis (n = 5) were stained using the 10-7G mAb; C and D: Lymphocytes infiltrating into inflammatory sites of the mucosal layer (C) and lymph nodules in intestinal tissues (D) of patients with Crohn’s disease (n = 5) were also stained. Photographs were acquired using a 20 × (upper) or 100 × (lower) objective. Scale bar, 100 µm. Positive staining was judged by comparison to the negative control. NC: Negative control.

Figure 3 Measurement of the serum 10-7G value and fucosylated prohaptoglobin amount in patients with inflammatory bowel disease. A: Serum 10-7G value of healthy volunteers and patients with ulcerative colitis, Crohn’s disease, or acute enteritis. Values are shown in relative units; B and C: Coefficients of correlation (B) between the 10-7G value and Fuc-pHpt amount and (C) between the 10-7G value and AAL-mHpt level. HV: Healthy volunteer; UC: Ulcerative colitis; CD: Crohn’s disease; AE: Acute enteritis.

Figure 4 Correlations between 10-7G values and clinical parameters in inflammatory bowel disease patients. A-C: In ulcerative colitis patients, the 10-7G value was negatively correlated with hemoglobin and positively correlated with C-reactive protein (CRP) level and full Mayo score; D and E: In Crohn’s disease patients, the 10-7G value was negatively correlated with albumin and positively correlated with CRP level; F: The 10-7G value was not correlated with the Crohn’s disease activity index. UC: Ulcerative colitis; CD: Crohn’s disease; Hb: Hemoglobin; CRP: C-reactive protein; Mayo: Mayo score; Alb: Albumin; CDAI: Crohn’s disease activity index.

Figure 5 Measurement of serum 10-7G values and correlation between 10-7G values and C-reactive protein in healthy volunteers who underwent a medical examination. A: The 10-7G value of healthy volunteers (HVs) who had elevated C-reactive protein (CRP) levels (> 0.3 mg/dL) diagnosed during routine medical examination (CRP-high HVs); B: Coefficient of correlation between the 10-7G value and CRP level in CRP-high HVs; C: 10-7G values of inflammatory bowel disease (IBD) patients with normal CRP level (≤ 0.3 mg/dL) (CRP-normal IBD) or high CRP level (> 0.3 mg/dL) (CRP-high IBD); D: Coefficient of correlation between the 10-7G value and CRP level in IBD patients. HV: Healthy volunteer; CRP: C-reactive protein; IBD: Inflammatory bowel disease; NS: Not significant.

Figure 6 Evaluation of the usefulness of the 10-7G value for pathologic diagnosis of ulcerative colitis compared to C-reactive protein and leucine-rich alpha-2 glycoprotein. A-C: Analysis of receiver operating characteristic curves for 10-7G value, leucine-rich alpha-2 glycoprotein, and C-reactive protein for differentiation between ulcerative colitis (UC) patients with endoscopic mucosal remission by Mayo endoscopic subscore (MES). AUC: Area under the curve; MES: Mayo endoscopic subscore; LRG: Leucine-rich alpha-2 glycoprotein; CRP: C-reactive protein.

Figure 7 Non-invasive follow-up chart of patients with ulcerative colitis using 10-7G value.