Basic Study
Copyright ©The Author(s) 2018.
World J Gastroenterol. Dec 21, 2018; 24(47): 5338-5350
Published online Dec 21, 2018. doi: 10.3748/wjg.v24.i47.5338
Figure 1
Figure 1 AGP01 cell line multicolor-FISH showing several chromosomal changes, including the monosomy of chromosome 12, where the PIWIL1 gene is located.
Figure 2
Figure 2 Electropherograms showing the insertion of seven adenines in the PIWIL1 gene sequence after using the CRISPR-Cas9 system (B) in comparison with the reference sequence of the negative control (A).
Figure 3
Figure 3 Amino acid sequences of the wild-type PIWIL1 protein and the PIWIL1 protein after the insertion of seven adenines by the CRISPR-Cas9 system.
Figure 4
Figure 4 Analysis of the migration capacity of the AGP01 cell line with and without PIWIL1 gene knockout. T0: Immediately after injury; T6: 6 h after injury; T12: 12 h after injury; T24: 24 h after injury. NC: Negative control. aP < 0.01. Two-way ANOVA, Bonferroni post-test. Photomicrography of AGP01 cell migration. A: Immediately after injury; B: 6 h after injury; C: 12 h after injury; D: 24 h after injury. The black lines represent approximation of the edges over time, demonstrating the migration capacity of the cells.
Figure 5
Figure 5 Analysis of the invasion capacity of the AGP01 cell line with and without the PIWIL1 gene knockout. Statistically significant difference between groups was shown by the Student’s t test (cP < 0.001). Photomicrography of the cell invasion assay demonstrating the decrease in the number of cells that invaded when PIWIL1 was knocked out. NC: Negative control.
Figure 6
Figure 6 Volcano plot comparing gene expression after PIWIL1 permanent knockout in the gastric cancer AGP01 cell line. Differentially expressed probes [adjusted P-value of < 0.05 and |Log2(Fold-Change)| > 1] are on superior left and right areas (red). mRNAs involved in invasion and migration processes are in green.