Overexpression of CREPT confers colorectal cancer sensitivity to fluorouracil

Figure 1 CREPT expression in tumor and adenoma tissues. A: Negative CREPT immunohistochemical staining in colorectal adenoma tissue; B: Positive CREPT immunohistochemical staining in CRC tissue; C: CREPT expression pattern in CRC sample; D: CREPT was determined by immunoblotting in CRC cell lines. CRC: Colorectal carcinoma.

Figure 2 CREPT expression in CRC correlates with clinicopathological characteristics. A: CREPT expression level correlates with pathological type and tumor differentiation; B: Survival curve of CRC patients shows significant difference between patients with or without 5-FU-bases adjuvant chemotherapy. CRC: Colorectal carcinoma.

Figure 3 Alteration of CREPT expression in CRC cells affects cell proliferation. A: Western blotting showed that expression of CREPT in SW620 and DLD1 changed after exposed to virus for 48 h; B and C: SW620 and DLD1 cells were incubated in 96-well plates, and CCK-8 assay determined cell viability; D: Cell cycle was detected by flow cytometry and indicated that CREPT accelerated cells through the G1/S check point. CRC: Colorectal carcinoma.

Figure 4 CREPT facilitates CRC cell response to 5-FU chemotherapy. A: Cells were treated with 5-FU (50 μg/mL), and CCK-8 assay estimated their viability; B: Annexin V-PI apoptosis detection show that DLD1 cells were less sensitive to 5-FU chemotherapy when CREPT was knocked down; C: SW620 and DLD1 cells were harvested after exposure to 5-FU (50 μg/mL) for 48 h, and western blotting showed that PARP was highly expressed in cells that expressed more CREPT. CRC: Colorectal carcinoma.