Basic Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Sep 14, 2017; 23(34): 6220-6230
Published online Sep 14, 2017. doi: 10.3748/wjg.v23.i34.6220
Figure 1
Open in New Tab Full Size Figure Download Figure
Figure 1 Herb-partitioned moxibustion treatment.
Figure 2
Open in New Tab Full Size Figure Download Figure
Figure 2 Comparison of the abdominal withdrawal reflex scores after treatment. A: AWR scores under 20 mmHg CRD stimulation; B: AWR scores under 40 mmHg CRD stimulation; C: AWR scores under 60 mmHg CRD stimulation; D: AWR scores under 80 mmHg CRD stimulation. bP < 0.01 vs normal group; dP < 0.01 vs model group; fP < 0.01 vs sham-HPM group; hP < 0.01 vs DMSO group. AWR: Abdominal withdrawal reflex; CRD: Colorectal distending; NG: Normal group; MG: Model group; SHPMG: Sham-HPM group; MIG: MEK-inhibitor group; DG: Dimethyl sulfoxide group; HPM: Herb-partitioned moxibustion.
Figure 3
Open in New Tab Full Size Figure Download Figure
Figure 3 Comparison of the mechanical withdrawal threshold and thermal withdrawal latency scores. A: MWT test used the Von Frey filament; B: TWL test followed Hargreaves. aP < 0.05 vs normal group; cP < 0.05 vs model group; eP < 0.05 vs sham-HPM group; gP < 0.05 vs DMSO group. MWT: Mechanical withdrawal threshold; TWL: Thermal withdrawal latency; NG: Normal group; MG: Model group; SHPMG: Sham-HPM group; MIG: MEK-inhibitor group; DG: Dimethyl sulfoxide group; HPM: Herb-partitioned moxibustion.
Figure 4
Open in New Tab Full Size Figure Download Figure
Figure 4 Hematoxylin-eosin staining of colonic mucosa. A: Normal group; B: Model group; C: HPM group; D: Sham-HPM group; E: MEK-inhibitor group; F: DMSO group (HE staining, × 200 magnification). HE: Hematoxylin-eosin; HPM: Herb-partitioned moxibustion; MEK: Mitogen-activated extracellular signal-regulated kinase; NG: Normal group; MG: Model group; SHPMG: Sham-HPM group; MIG: MEK-inhibitor group; DG: Dimethyl sulfoxide group.
Figure 5
Open in New Tab Full Size Figure Download Figure
Figure 5 Comparison of expression of phospo-mitogen-activated extracellular signal-regulated kinase 1, phosphor-extracellular signal-regulated kinase 1/2 and cAMP response element binding protein in rat spinal cord. A: Protein expression of pMEK1; B: Protein expression of pERK1/2; C: Protein expression of pCREB. bP < 0.01 vs normal group; dP < 0.01 vs model group; fP < 0.01 vs sham-HPM group; hP < 0.01 vs DMSO group. pCREB: cAMP response element binding protein; DMSO: Dimethyl sulfoxide; pERK: Phosphor-extracellular signal-regulated kinase; HPM: Herb-partitioned moxibustion; pMEK1: Phospo-mitogen-activated extracellular signal-regulated kinase 1; NG: Normal group; MG: Model group; SHPMG: Sham-HPM group; MIG: MEK-inhibitor group; DG: Dimethyl sulfoxide group.
Figure 6
Open in New Tab Full Size Figure Download Figure
Figure 6 Comparison of expression of mitogen-activated extracellular signal-regulated kinase, extracellular signal-regulated kinase and cAMP response element binding protein mRNAs in rat spinal cord. A: mRNA expression of MEK; B: mRNA expression of ERK; C: mRNA expression of CREB. bP < 0.01 vs normal group; dP < 0.01 vs model group; fP < 0.01 vs sham-HPM group; hP < 0.01 vs DMSO group. CREB: cAMP response element binding protein; DMSO: Dimethyl sulfoxide; ERK: Extracellular signal-regulated kinase; HPM: Herb-partitioned moxibustion; MEK: Mitogen-activated extracellular signal-regulated kinase; NG: Normal group; MG: Model group; SHPMG: Sham-HPM group; MIG: MEK-inhibitor group; DG: Dimethyl sulfoxide group.
Figure 7
Open in New Tab Full Size Figure Download Figure
Figure 7 Flow chart of the study design and treatment. AWR: Abdominal withdrawal reflex: CREB: cAMP response element binding protein; ERK: Extracellular signal-regulated kinase; DG: DMSO group; HE: Hematoxylin-eosin; HPM: Herb-partitioned moxibustion; MEK: Mitogen-activated extracellular signal-regulated kinase; MIG: MEK-inhibitor group; MG: Model group; MWT: Mechanical withdrawal threshold; NG: Normal group; SD: Sprague-Dawley; SHPMG: Sham-HPM group; TWL: Thermal withdrawal latency.