Apoptosis induced by a low-carbohydrate and high-protein diet in rat livers

Figure 1 Flow cytometry in hepatocytes from the experimental diet group and control diet group at 8 wk. Annexin-V was used to identify late apoptosis and non-apoptotic death and propidium iodide for early apoptosis. A-% early apoptosis; B-% non-apoptotic death; C-% late apoptosis; D-% viable cells. EDG: Experimental diet group; CDG: Control diet group.

Figure 2 Staining of liver fragments at 8 wk: experimental diet group and control diet group. A: Normal central vein, functional sinusoidal dilation, and small hepatocytes in the EDG (HE, original magnification 400 ×); B: Normal central vein, sinusoidal cells, and hepatocytes in the CDG (HE, original magnification 400 ×); C: Zones without glycogen in the EDG (HE, original magnification 400 ×); D: Homogeneous distribution of glycogen in the CDG (HE, original magnification 400 ×). EDG: Experimental diet group; CDG: Control diet group; HE: Hematoxylin and eosin.