Purification and activity of human fetal hepatic stimulating substance

Figure 1 Nondenaturing gradient PAGE. Sample: DEAE-Sephadex A50 peak V.

Figure 2 The sample was prepared in 0. 1% SDS and SDS-PAGE was run. Silver staining was performed. Lane 1 is molecular weight standards: A = 18 kDa; B = 34 kDa; C = 53 kDa; D = 76 kDa; E = 116 kDa; F = 170 kDa and G = 212 kDa. Lane 2 is major bands of nondenaturing PAGE.

Figure 3 After ISCO concentrator electrophoresis, the activity showed: A: the protein bands on the tube gel; B: the content of hHSS by ELISA method; C: the value of activity (IS) by ³H-TdR incorporation method.