Dubin-Johnson syndrome coinciding with colon cancer and atherosclerosis

Figure 1 Histochemistry and immunohistology of the liver in Dubin-Johnson syndrome patient. A: Accumulation of distinctive dark brown pigment in hepatocytes was detected in hematoxylin and eosin staining; B: The pigment was negative in Perls reaction; C: The pigment reduced Masson’s solution; D: Immunohistochemical analysis for ABCC2/MRP2 protein was negative compared to the positive control (inset). Original magnification ×400 (bars correspond to 100 μm).

Figure 2 Mutation analysis results. A: Presence of a heterozygous deletion c.2360_2366delCCCTGTC in exon 18 of ABCC2 (panel A, middle lane) was confirmed by sequence analysis of individual alleles separated by cloning (wildtype allele - panel A, upper lane; mutated allele - panel A, lower lane); B: In addition, a heterozygous splice site mutation c.3258+1G>A was detected in intron 23; C: Presence of the latter mutation was confirmed by polymerase chain reaction-Bsh1236I restriction fragment length polymorphism analysis.