Metabonomic studies of pancreatic cancer response to radiotherapy in a mouse xenograft model using magnetic resonance spectroscopy and principal components analysis

Figure 1 Effect of radiotherapy on the growth of human pancreatic tumor in nude mouse. Mice received a subcutaneous injection of SW1990 cells. When the tumor volume reached about 800 mm³, the mice were divided into four groups. Group A mice were used as untreated controls. Groups B, C, and D received 10, 20, and 30 Gy radiation doses, respectively. Tumor size was measured for two weeks. ^aP < 0.05 vs the untreated group.

Figure 2 High-resolution magic angle spinning proton magnetic resonance spectroscopy spectra of normal pancreas and transplanted pancreatic tumor (500 mHz). A: Normal pancreas (a); Transplanted pancreatic tumor (b); Amplified data from spectra region δ3.30-3.15 (c); Amplified data from spectra region δ3.15-3.48 (d). For peak assignments, see list of abbreviations used; B: Principal Component Analysis to compare the metabolic profiles between normal pancreas and pancreatic cancer based on the high-resolution magic angle spinning proton magnetic resonance spectroscopy spectra. Panels (a) and (b) are score and loading plots. ❍: Normal pancreas; ∆: Pancreatic cancer.

Figure 3 High-resolution magic angle spinning proton magnetic resonance spectroscopy spectra of transplanted pancreatic tumor after radiotherapy (500 mHz). A: Untreated group(a); 10 Gy treatment group (b); 20 Gy treatment group (c); 30 Gy treatment group (d); B: Principal component analysis to compare the metabolic profiles of the pancreatic tumor after radiotherapy based on the high-resolution magic angle spinning proton magnetic resonance spectroscopy spectra. Panels (a) and (b) are scores and loadings plots. ❍: Untreated group; ∆: 10 Gy treatment group; ×: 20 Gy treatment group; +: 30 Gy treatment group.