Promising effect of Magliasa, a traditional Iranian formula, on experimental colitis on the basis of biochemical and cellular findings

Figure 1 Microscopic characteristics of the herbal ingredients of Magliasa. A: Lepidium sativum seed. A1: Pericarp; A2 and A3: Sclereids of the mesocarp; A4: Parenchyma of the endosperm; B: Linum usitatissimum seed. B1: Endosperm; B2: Epidermis; B3: Fiber; B4: Sclerenchyma; B5: Parenchyma of the testa; B6: Pigment layer of testa; C: Terminalia chebula fruit. C1: Epidermis; C2: Fiber; C3: Parenchyma of the mesocarp; C4 and C5: Sclereids; C6: Vessels; D: Allium ampeloprasum cv. Porrum seed. D1: Endosperm; D2: Mesoderm; D3: Epidermis of the testa; E: Bunium persicum fruit. E1: Endocarp; E2 and E3: Endosperm; E4 and E5: Sclereids of the mesocarp; E6: Vessels; E7: Vittae. Magnification of all images was 40.

Figure 2 Histological images of colon samples. In the Sham group, colons were within normal limits including crypts (A) and submucosal tissue (B), but intense transmural inflammation (C), and severe crypt destruction (D) were observed in the control group. Moderate crypt distortion (E and G) and inflammatory cell infiltration (F and H) was seen in the Mag-5 and Mag-100 groups. Mild focal inflammation and crypt abscess were observed in the Mag-20 and infliximab groups (I and J). Mild focal inflammation was observed in the Dexa group (K). Magnification of all images was 100. Dexa: Dexamethasone; Inf: Infliximab; Mag-50: Magliasa at a dose of 50 mg/kg; Mag-100: Magliasa at a dose of 100 mg/kg; Mag-200: Magliasa at a dose of 200 mg/kg.

Figure 3 Levels of different biochemical parameters in the colon of rats after 2 wk of treatment. A: Tumor necrosis factor-alpha (TNF-α); B: Interleukin-1 beta (IL-1β); C: Total antioxidant capacity as a ferric reducing antioxidant power (FRAP) level; D: Lipid peroxidation as a thiobarbituric acid reactive substances (TBARS) level; E: Neutrophil infiltration as myeloperoxidase (MPO) activity. Values are mean ± SE. ^aP < 0.05 vs the Sham group; ^cP < 0.05 vs the control group; ^eP < 0.05 vs the dexamethasone group. Dexa: Dexamethasone; Inf: Infliximab; Mag-50: Magliasa at a dose of 50 mg/kg; Mag-100: Magliasa at a dose of 100 mg/kg; Mag-200: Magliasa at a dose of 200 mg/kg.