Effects of warm ischemia time on biliary injury in rat liver transplantation

Figure 1 With prolongation of secondary biliary warm ischemia time, the number of proliferating-cell nuclear antigen-positive cholangiocytes was significantly reduced. A: Proliferating-cell nuclear antigen (PCNA)-positive cholangiocytes in group II at 24 h after hepatic artery (HA) reperfusion; B: PCNA-positive cholangiocytes in group IV at 24 h after HA reperfusion. A and B, original magnification × 400.

Figure 2 With prolongation of secondary biliary warm ischemia time, more biliary epithelial cells became apoptotic. A: Biliary epithelial cell apoptosis in group II at 24 h after hepatic artery (HA) reperfusion; B: Biliary epithelial cell apoptosis in group IV at 24 h after HA reperfusion. A and B, original magnification × 400.

Figure 3 Histological examination of the liver at 24 h after hepatic arterial reperfusion. A: Cholangiocyte injury can be found in group I; B: More marked injury occurs in group IV. A and B: Hematoxylin-eosin, original magnification × 400.