Emodin promoted pancreatic claudin-5 and occludin expression in experimental acute pancreatitis rats

Figure 1 Effects of emodin on pancreatic injury in sham group (A), pancreatitis group (B), emodin group (C) and (D) histological score. (Original magnification, × 200). Six rats were studied in each experimental group at each time point. Results are mean ± SD. ^aP < 0.05 vs sham group; ^cP < 0.05 vs pancreatitis group.

Figure 2 Effects of emodin on pancreatic (A) tumor necrosis factor-α, (B) interleukin-6, (C) edema and (D) dye extravasation. Six rats were studied in each experimental group at each time point. Results are mean ± SD. ^aP < 0.05 vs sham group; ^cP < 0.05 vs pancreatitis group. TNF-α: Tumor necrosis factor-α; IL-6: Interleukin-6.

Figure 3 Immunohistochemical staining of claudin-5 (A-C) and occludin (D-F) in sham group (A, D), pancreatitis group (B, E) and emodin group (C, F) (original magnification, × 200).

Figure 4 Effects of emodin on claudin-5 (A, C) and occludin (B, C) protein levels in rats. Six rats were studied in each experimental group at each time point. Results are expressed as mean ± SD. ^aP < 0.05 vs sham group; ^cP < 0.05 vs pancreatitis group. GAPDH: Glyceraldehyde phosphate dehydrogenase.

Figure 5 Effects of emodin on claudin-5 (A) and occludin (B) mRNA levels in rats. Six rats were studied in each experimental group at each time point. Results are expressed as mean ± SD. ^aP < 0.05 vs sham group; ^cP < 0.05 vs pancreatitis group. GAPDH: Glyceraldehyde phosphate dehydrogenase.