Subcellular distribution of nitric oxide synthase isoforms in the rat duodenum

Figure 1 Postembedding immunogold labeling for neuronal nitric oxide synthase in a myenteric ganglion of the rat duodenum. The majority of the gold particles are above the structureless cytoplasm (arrows). M: Mitochondria

Figure 2 Postembedding immunogold labeling for neuronal nitric oxide synthase in an enteric smooth muscle cell. Gold particles coding for neuronal nitric oxide synthase accumulated in the mitochondria (M, encircled). Only a few gold particles are seen above the structureless cytoplasm (arrows).

Figure 3 Postembedding immunogold labeling for neuronal nitric oxide synthase in the capillary endothel in the vicinity of the myenteric plexus of the rat duodenum. The majority of the gold particles are bound to the vesicular membranes or are situated inside vesicles (arrows). L: capillary lumen

Figure 4 Postembedding immunogold labeling for endothelial nitric oxide synthase in a myenteric ganglion of the rat duodenum. The majority of the gold particles are distributed evenly (arrows); however, mitochondrial accumulation of endothelial nitric oxide synthase-coding gold particles (M, encircled) is also observed.

Figure 5 Postembedding immunogold labeling for endothelial nitric oxide synthase in an enteric smooth muscle cell. Gold particles are evenly distributed (arrows) and avoid caveolae.

Figure 6 Postembedding immunogold labeling for endothelial nitric oxide synthase in an endothelial cell. The gold particles are bound to the vesicular membranes or are situated inside vesicles (arrows).

Figure 7 Postembedding immunogold labeling for inducible nitric oxide synthase. Gold particles (encircled) are randomly distributed in the enteric muscle cells (SM) and also in the myenteric neurons (N). M: Mitochondria.

Figure 8 Postembedding immunogold labeling for inducible nitric oxide synthase in the endothelial cell of a capillary in the vicinity of the myenteric plexus of the rat duodenum. The majority of the gold particles were bound to the vesicular membranes or are situated inside vesicles (arrows).