Original Article
Copyright ©2010 Baishideng.
World J Gastroenterol. Feb 21, 2010; 16(7): 854-861
Published online Feb 21, 2010. doi: 10.3748/wjg.v16.i7.854
Figure 1
Figure 1 Representative microphotographs of the colon sections stained with hematoxylin and eosin (original magnification, × 100). A: Control colon; B: Colon was obtained on day 3 after intrarectal TNBS.
Figure 2
Figure 2 IL-1β (A), IL-6 (B), TNF (C), IFN-γ (D) mRNA and miRNA155 (E) expressions in colon homogenates of control and TNBS colitis groups. qPCR-derived IL-1β, IL-6, TNF, IFN-γ, and miRNA155 expressions in colon homogenates were significantly increased in TNBS-induced colitis. aP < 0.05 vs control.
Figure 3
Figure 3 TNF mRNA (A) and miRNA155 (B) expressions in LNs and CD4+ T cells in control and TNBS colitis groups. TNF mRNA and miRNA155 expressions in LNs and CD4+ T cells were significantly increased in TNBS-induced colitis. aP < 0.05 vs control.
Figure 4
Figure 4 TNF mRNA (A) and miRNA155 (B) expressions in CD4+ T cells cultured with or without anti-CD3/CD28 antibody, and the TNF protein expression (C) in the supernatants of CD4+ T cells culture media with or without anti-CD3/CD28 antibody. TNF mRNA and miRNA155 expressions in CD4+ T cells cultured with anti-CD3/CD28 antibody, and the TNF concentration in the supernatants of culture media containing anti-CD3/CD28 antibody increased in comparison to those in cells or media cultured without anti-CD3/CD28 antibody. aP < 0.05 vs control.
Figure 5
Figure 5 Relationships between miRNA155 expression in CD4+ T cells and TNF mRNA expression in CD4+ T cells (A) or TNF concentration in CD4+ T cells culture media (B). Data analyzed by Pearson’s correlation coefficient.