Redefining the properties of an osmotic agent in an intestinal-specific preservation solution

Figure 1 Infiltration of fluorescein isothiocyanate-labeled dextrans during cold ischemic storage. All magnifications (× 10 objective) are of representative fluorescent intensities and mucosal injury. All photos have a standardized exposure time of 250 ms for the fluorescein isothiocyanate channel; exposure times for 4',6-diamidino-2-phenylindole, a common nuclear stain, are 2-20 ms and are included for contrast.

Figure 2 Histology after 12 h cold ischemic storage. Photos are of representative median grades of histologic injury according to a modified Park’s classification of intestinal injury[25]. Magnification with 10 × objective. HE staining, 5 μm sections. D1: Grade 6.5; D2: Grade 6.5; D3: Grade 7.5; D4: Grade 7; D5: Grade 4.

Figure 3 Parameters of energy metabolism during cold ischemic storage. Adenosine triphosphate (ATP), total adenylates and energy charge levels are presented. ^a,b,c,dP < 0.05, significantly different vs D1, D2, D3, D4, respectively.

Figure 4 Correlation between dextran size and energy metabolism. Linear regression analysis was performed; correlation coeffients (r²) are presented. ATP: Adenosine triphosphate; Mw: Molecular weight.