Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation

Figure 1 Detection sensitivity. RT-PCR detection of HT-29 cells in 1 x 10⁶ hepatocytes. Lane M: 100 bp ladder (100 bp to 1 kb); Lane β: β-actin; Lane 1: 100 HT-29 cells; Lane 2: 10 HT-29 cells; Lane 3: 5 HT-29 cells; Lane 4: 1 HT-29 cell; Lane 5: Hepatocytes only; Lane 6: HT29 only.

Figure 2 Tumor-purging efficacy. RT-PCR detection of EpCAM RNA in HT-29-cell-spiked hepatocytes (1 x 10⁶) with and without treatment with Ber-EP4-coated immunomagnetic beads. Lane M: 100 bp ladder (100 bp to 1 kb); Lane β: β-actin; Lane 1: 50 000 HT-29 cells; Lane 2: 50 000 HT-29 cells treated with immunomagnetic beads; Lane 3: 10 000 HT-29 cells; Lane 4: 10 000 HT-29 cells treated with immunomagnetic beads; Lane 5: 10 HT-29 cells treated with immunomagnetic beads; Lane 6: 10 HT-29 cells only.