Combination of small interfering RNAs mediates greater suppression on hepatitis B virus cccDNA in HepG2.2.15 cells

Figure 1 Relative expression of HBsAg (A) and HBeAg (B). ^aP < 0.05 vs siRNA1 or siRNA2.

Figure 2 Reduction in HBV mRNA level after treatment with siRNA. M: marker, lane 1: HK, lane 2: psil/U6, lane 3: siRNAs 1 and 2, lane 4: siRNA2, lane 5: siRNA1.

Figure 3 Inhibition of HBV DNA (A) and cccDNA (B) amplification after treatment with siRNA. ^aP < 0.05 vs siRNA1 or siRNA2.

Figure 4 Dose-dependent inhibitory effects of siRNA on HBV antigen HBsAg expression (A) and HBV replication (B). ^aP < 0.05 vs 2 &mgr;g or 3 &mgr;g.