ShRNA-mediated gene silencing of β-catenin inhibits growth of human colon cancer cells

Figure 1 Identification of plasmid Pgenesil by restriction enzyme digestion. Lane 1: Marker; Lane 2: Plasmid Pgenesil-CAT1; Lane 3: Plasmid Pgenesil-CAT1 digested by SalI; Lane 4: Plasmid Pgenesil-CAT2; Lane 5: plasmid Pgenesil-CAT2 digested by SalI; Lane 6: Plasmid Pgenesil-Negative; Lane 7: Plasmid Pgenesil-Negative digested by SalI.

Figure 2 RNA interference-mediated suppression of cellular mRNA. A: The mRNA expressions of different goal genes in colon cancer cells at 48 h post-transfection were examined by semiquantitive RT-PCR. The β-actin gene served as internal control. Lane 1: Blank control; Lane 2: Negative control; Lane 3: Pgenesil-CAT; Lane 4: Pgenesil-CAT2; B: The inhibition rates of different genes down-regulated by plasmid Pgenesil-CAT1 and Pgenesil-CAT2. Goal gene 1: β-catenin; 2: c-myc; 3: cyclinD1.

Figure 3 RNA interference-mediated suppression of cellular protein. A: Western blot analysis for different goal genes. The β-actin gene served as internal control. Lane 1: Blank control; Lane 2: Negative control; Lane 3: Pgenesil-CAT1; Lane 4: Pgenesil-CAT2 group; B: The inhibition rates of different genes down-regulated by plasmids Pgenesil-CAT1 and Pgenesil-CAT2. Goal gene 1: β-catenin; 2: c-myc; 3: cyclinD1.