Rapid Communication
Copyright ©2007 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 14, 2007; 13(46): 6219-6225
Published online Dec 14, 2007. doi: 10.3748/wjg.v13.i46.6219
Figure 1
Figure 1 The effect of a mixture of inflammatory cytokines on NAT1 activity in KKU-100 cells. Assays were performed with the cytosol fraction extracted from KKU-100 cells and treated with a combination of cytokines for 48 h. A: Kinetic analysis of NAT1 activity; B: Activity of NAT1 at various substrate concentrations (PABA) of KKU-100 cells with or without treatment with cytokines. aP < 0.05 between cytokine treated and control groups.
Figure 2
Figure 2 Oxidant status of KKU-100 cells after exposure to a mixture of inflammatory cytokines. A: Stimulation of nitric oxide production, assayed as nitrite levels. Cultured media was collected at 24 and 48 h after exposure; B: Superoxide formation. Cell cultures were washed and incubated with PMA (0.68 μg/mL), L-NAME (100 μmol/L), or NADPH (200 μmol/L), and superoxide production was measured using the chemiluminescence method; C: Redox status was assessed as the GSH and GSSG ratio. Results are presented as mean ± SE from 3 separate experiments. aP < 0.05 between cytokine treated and the respective control groups.
Figure 3
Figure 3 Effect of treatment with the nitric oxide donor GSNO on KKU-100 cells. Cell cultures were treated with 100 μmol/L GSNO for 2 and 48 h. A: Total GSH in the cells; B: GSH/GSSG ratio; C: Activity of NAT1 PABA-acetylation. Bars represent the mean ± SEM from 3 separate experiments. aP < 0.05 vs control groups, cP < 0.05 vs the 48-h treatment group.
Figure 4
Figure 4 Effect of the cytokine mixture and a nitric oxide donor on the expression of NAT1 in KKU-100 cells. KKU100 cells were incubated with a mixture of cytokines for 48 h or with 100 μmol/L GSNO for 2-48 h. Cells were harvested, and RNA was extracted and analyzed by reverse transcription PCR using FDFT1 as an internal control. aP < 0.05 vs controls.