Basic Research
Copyright ©2007 Baishideng Publishing Group Inc.
World J Gastroenterol. Oct 21, 2007; 13(39): 5232-5237
Published online Oct 21, 2007. doi: 10.3748/wjg.v13.i39.5232
Figure 1
Figure 1 Transfection of recombination plasmids into cultured (A) and non-cultured (B) pancreatic ductal epithelial cells.
Figure 2
Figure 2 XlHbox8 mRNA expression 24 h after transfection of recombination plasmids.
Figure 3
Figure 3 PDX-1 and insulin mRNA expression after transfection of recombination plasmids.
Figure 4
Figure 4 Western blot analysis of PDX-1 and insulin protein showing the increased expression of PDX-1 (P < 0. 01) and insulin (P < 0.01) protein in the transfected cells. C0-C28: Untransfected group on d 0-28; M0-M28: Transfected group on d 0-28; UNT-I: Insulin expression in untransfected group; UNT-P: PDX-1 expression in untransfected group; TRA-I: Insulin expression in transfected group; TRA-P: PDX-1 expression in transfected group.
Figure 5
Figure 5 Dithizone staining of insulin-producing cells in untransfected group (A) and transfected group (B).
Figure 6
Figure 6 Response of insulin secretion to physiological stimuli. Insulin secretion was significantly increased in the transfected group compared with the untransfected group (bP < 0.01).