Apoptotic effect of Epigallocatechin-3-gallate on the human gastric cancer cell line MKN45 via activation of the mitochondrial pathway

Figure 1 The change of apoptotic cells ratio of MKN45 cells after being treated with caspase-3 inhibitor detected by Annexin V-FITC + PI dual staining.

Figure 2 Caspase-3 activity and its relationship with the time and dose of EGCG treatment.

Figure 3 The relationship between the absorbance of Rhodamine123 and time and dose of EGCG treatment. The mitochondrial membrane potential was measured by the absorbance of Rhodamine 123.

Figure 4 RT-PCR and western blot analysis of apoptosis-related genes. The mRNAs and proteins expression of Bcl-2 family was detected by RT-PCR and western blot at indicated time in presence of 80 μmol/L EGCG insult. β-actin served as the internal control.