Expression of SNC73, a transcript of the immunoglobulin α-1 gene, in human epithelial carcinomas

Figure 1 Original expression of SNC73 in human epithelial-derived tumor cell lines. (A) RT-PCR showing the expression of SNC73 in five cell lines, M: DL2000 Marker, 1. LoVo, 2. SW480, 3. HeLa, 4. SMC7721, 5. Bcap37. (B, C, D and E) immunohistochemistry showing the expression of SNC73 protein, of IgHα1. of Igκ. and of Igλ, respectively.

Figure 2 Expression of SNC73 mRNA in the epithelial cells of colon mucosa. A, C: In situ hybridization of SNC73 mRNA in the normal colon mucosa and colon carcinoma by anti-sense SNC73 RNA probe, respectively; B, D: In situ hybridization of SNC73 mRNA in the normal colon mucosa and colon carcinoma by SNC73 sense RNA probe, respectively, as negative control; E: Expression of cytokeratin (Rhodamine-labeled) in epithelial cells by immunohistochemistry; F: Expression of IgHa1 (FITC-labeled) was expressed in epithelial cells by immunohistochemistry; G: The merger of (E) and (F) × 200.

Figure 3 The V(D)J recombination machine is present in human epithelia-derived tumor cell lines. A-E: RT-PCR analysis of RAG1, RAG2, EBF, E2A, Pax5 in five cell lines. M: DL2000 Marker, 1. LoVo, 2. SW480, 3. HeLa, 4. SMC7721, 5. Bcap37; F: Immunoblot showing the expression of RAG1 and RAG2 in five cell lines.