Differences in expression of retinal proteins between diabetic and normal rats

Figure 1 2-DE map of proteins in rat retinae. 2-DE was performed on an immobilized 17 cm pH 5-8 strip, followed by the second-dimensional separation on 12% poly-acrylamide gels. The separated proteins were stained with silver nitrate.

Figure 2 Types of protein expression patterns obtained from normal and diabetic retinal tissues and proteins pertaining to each pattern. Norm Qty on the Y-axis stands for normalized quantity, D and N on the X-axis stand for diabetic and normal retinal tissue, respectively. The 8 proteins visualized by MS/MS were divided by expression pattern into pattern 1 through 3 and displayed by protein spots in 2-D gel.