In vitro porcine brain tubulin assembly inhibition by water extract from a Chinese medicinal herb, Tripterygium hypoglaucum Hutch

Figure 1 Scheme of in vitro tubulin assay. The tubulin assembly was achieved at the end of the absorption after 20 min incubation and kept a steady state level at 37 °C. The disassembly was carried out at 4 °C and after the assembly (40-min incubation).

Figure 2 Inhibition of in vitro tubulin assembly by COL at the concentration of ■:0. 0025 mmol/L,▲:0.0050 mmol/L,▼:0.0075mmol/L (A) and THH at the concentration of ■:0.05mg/mL,▲:0.07g/mL,▼:0.09mg/mL (B). ●: Solvent control