Published online Feb 21, 2006. doi: 10.3748/wjg.v12.i7.1133
Revised: July 29, 2005
Accepted: December 19, 2005
Published online: February 21, 2006
AIM: To investigate the effect of Tripterygium hyp-oglaucum Hutch (THH) on the assembly and disassembly process of tubulin and its possible mode of action.
METHODS: In vitro porcine brain tubulin assembly assay was employed to analyze the inhibitory effects of THH at different concentrations (0.05 μg/L, 0.07 μg/L, 0.09 μg/L). Colchicine (0.0025 mmol/L, 0.0050 mmol/L, 0.0075 mmol/L) was used as a positive control.
RESULTS: THH could significantly inhibit the assembly of isolated porcine brain tubulin at all tested concentrations.
CONCLUSION: THH is capable of inducing aneuploidy in mammals via tubulin polymerization inhibition pathway and may pose a genetic risk to human beings.