Expression patterns of cytokine, growth factor and cell cycle-related genes after partial hepatectomy in rats with thioacetamide-induced cirrhosis

Figure 1 Hepatic A: TNF-α and B: IL-6 concentrations after PH on healthy rats (white bars) and cirrhotic rats (black bars). At different time points after the operation, the remnant liver was harvested, homogenized and the supernatant was used for analysis. For each time point, the supernatant from 6 animals was pooled and used for ELISA analysis. All analyses were carried out in triplicates and the results are expressed as mean + SD. ^cP < 0.05, ANOVA analysis, for comparison between control rats before (at 0 h) and after PH. ^aP < 0.05, ANOVA analysis, for comparison between the cirrhotic group and the corresponding control group at each time point.

Figure 2 Hepatic A: HGF, B: TGF-α and C: 28S rRNA expressions after PH on healthy rats (white bars) and cirrhotic rats (black bars). At different time points after the operation, the remnant liver was harvested, and RNA was extracted. For each time point, the RNA from 6 animals was pooled and used for reverse-transcription followed by PCR. The PCR product was separated by agarose-gel electrophoresis and visualized by ethidium-bromide staining. The gel image was captured and the bands were quantified using the Analytical Imaging Station software. All analyses were carried out in triplicates. A representative gel (C, control, healthy rats; E, cirrhotic rats; the number indicates the time post-PH in h) and the quantification analysis expressed as mean + SE (AU = arbitrary units) are shown. ^cP<0.05, ANOVA analysis, for comparison between control rats before (at 0 h) and after PH. ^aP < 0.05, ANOVA analysis, for comparison between the cirrhotic group and the corresponding control group at each time point.

Figure 3 Effect of PH on cyclin expressions in healthy rats (white bars) and cirrhotic rats (black bars). At different time points after the operation, the remnant liver was harvested, homogenized and the supernatant was used for analysis. For each time point, the supernatant from 6 animals was pooled and used for Western blot analysis. The blot image was captured and the bands were quantified using the Analytical Imaging Station software. All analyses were carried out in triplicates. A representative blot (C, control, healthy rats; E, cirrhotic rats; the number indicates the time post-PH in h) and the quantification analysis expressed as mean+SE (AU = arbitrary units) are shown. A: Cyclin D1 expression. B: Cyclin D3 expression. C: Cyclin E expression. D: Cyclin A expression. ^cP < 0.05, ANOVA analysis, for comparison between control rats before (at 0 h) and after PH. ^aP < 0.05, ANOVA analysis, for comparison between the cirrhotic group and the corresponding control group at each time point.

Figure 4 Effect of PH on cyclin-dependent kinase expressions in healthy rats (white bars) and cirrhotic rats (black bars). At different time points after the operation, the remnant liver was harvested, homogenized and the supernatant was used for analysis. For each time point, the supernatant from 6 animals was pooled and used for Western blot analysis. The blot image was captured and the bands were quantified using the Analytical Imaging Station software. All analyses were carried out in triplicates. A representative blot (C, control, healthy rats; E, cirrhotic rats; the number indicates the time post-PH in h) and the quantification analysis expressed as mean+SE (AU = arbitrary units) are shown. A: Expression of cdk4. B: Expression of cdk2. ^cP < 0.05, ANOVA analysis, for comparison between control rats before (at 0 h) and after PH. ^aP < 0.05, ANOVA analysis, for comparison between the cirrhotic group and the corresponding control group at each time point.

Figure 5 Hepatic A: p21, B: p27 and C: p53 expressions after PH on healthy rats (white bars) and cirrhotic rats (black bars). At different time points after the operation, the remnant liver was harvested, and RNA was extracted. For each time point, the RNA from 6 animals was pooled and used for reverse-transcription followed by PCR. The PCR product was separated by agarose-gel electrophoresis and visualized by ethidium-bromide staining. The gel image was captured and the bands were quantified using the Analytical Imaging Station software. All analyses were carried out in triplicates. A representative gel (C, control, healthy rats; E, cirrhotic rats; the number indicates the time post-PH in h) and the quantification analysis expressed as mean + SE (AU = arbitrary units) are shown. ^cP< 0.05, ANOVA analysis, for comparison between control rats before (at 0 h) and after PH. ^aP < 0.05, ANOVA analysis, for comparison between the cirrhotic group and the corresponding control group at each time point.