Expression patterns of cytokine, growth factor and cell cycle-related genes after partial hepatectomy in rats with thioacetamide-induced cirrhosis

Figure 2 Hepatic A: HGF, B: TGF-α and C: 28S rRNA expressions after PH on healthy rats (white bars) and cirrhotic rats (black bars). At different time points after the operation, the remnant liver was harvested, and RNA was extracted. For each time point, the RNA from 6 animals was pooled and used for reverse-transcription followed by PCR. The PCR product was separated by agarose-gel electrophoresis and visualized by ethidium-bromide staining. The gel image was captured and the bands were quantified using the Analytical Imaging Station software. All analyses were carried out in triplicates. A representative gel (C, control, healthy rats; E, cirrhotic rats; the number indicates the time post-PH in h) and the quantification analysis expressed as mean + SE (AU = arbitrary units) are shown. ^cP<0.05, ANOVA analysis, for comparison between control rats before (at 0 h) and after PH. ^aP < 0.05, ANOVA analysis, for comparison between the cirrhotic group and the corresponding control group at each time point.