Folate levels in mucosal tissue but not methylenetetrahydrofolate reductase polymorphisms are associated with gastric carcinogenesis

Figure 1 A: HinfIdistinguishes MTHFR 677 (C→T) PCR products. M: 50 bp Ladder; L1, 2, 5 and 9: CT genotype; L3, 4 and 6: CC genotype; L7 and 8: TT genotype; B: Mbo II distinguishes MTHFR (A→C) PCR products. M: 50 bp ladder; L1: CC genotype; L2 and 3: AC genotype; L4-9: AA genotype.

Figure 2 Methylation-specific PCR (MSP) for tumor-related genes. MSP was performed on DNA from gastric cancerous tissue with primers designed to specifically detect methylated and unmethylated promoter regions. The data shown are representative of three replicate MSP experiments. Marker: 100 bp ladder; U: Unmethylated-MSP; M: Methylated-MSP.