Viral Hepatitis
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 7, 2006; 12(33): 5301-5305
Published online Sep 7, 2006. doi: 10.3748/wjg.v12.i33.5301
Figure 1
Figure 1 Sensitivity and specificity of the YMDD mutant detection by mutant-specific PCR. A: Various copies of wild-type sequence, YVDD mutant sequence, and mix of both sequences at the indicated concentrations. The left most lane contains molecular weight marker in base pair unit. The detection limit was 101 copies of YVDD mutant in 108 copies of wild-type HBV or 104 copies of YVDD mutant in 109 copies of wild-type HBV; B: Various copies of wild-type sequence, YIDD mutant sequence, and mix of both sequences at the indicated concentrations. The left most lane contains molecular weight marker in base pair unit. The detection limit was 101 copies of YIDD mutant in 108 copies of wild-type HBV or 103 copies of YIDD mutant in 109 copies of wild-type HBV.