Recombinant hybrid protein, Shiga toxin and granulocyte macrophage colony stimulating factor effectively induce apoptosis of colon cancer cells

Figure 1 Effect of different concentrations of StxA1-GM-CSF on LS174T cell line by MTT assay. (^aP  <  0.01, ^bP  <  0.001 vs trypan blue exclusion assay).

Figure 2 Morphology of normal cells (A) and apoptotic cells (B) after treatment with hybrid protein.

Figure 3 Hoechst 33342-staining of LS174T cells treated with StxA1-GM-CSF. A: control; B: cells after treatment.

Figure 4 Apoptosis percentage in the presence of 40 ng/mL StxA1-GM-CSF at different time intervals (A), ^aP < 0. 05, ^bP < 0.001. Apoptosis percentage in the presence of different concentration of StxA1-GMCSF for 6 h (B). ^dP <  0.01, ^fP <  0.001.