Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721

Figure 1 Degested results of the empty rector pSuNeo and recombinant pSuNeo-SVV. A: Electrophoretic profile of pSuNeo-SVV digested with restriction enzymes. Lane 1: DNA marker DL-15000, lane 2: pSuNeo, lane 3: pSuNeo/Sal I, lane 4: pSuNeo-SVV, lane 5: pSuNeo-SVV/Sal I, lane 6: pSuNeo-SVV/Sca I. B: Electrophoretic profile survivin gene by RT-PCR. Lane 1: DNA marker DL-2000, lane 2: untransfected survivin gene, lane 3: pSuNeo, lane 4: pSuNeo-SVV, lane 5: empty control, lane 6: β-actin.

Figure 2 Immunohistochemical staining of survivin in SMMC-7721 cells. A: SMMC-7721 cells, B: SMMC-7721 cells transfected with pSuNeo, C: SMMC-7721 cells transfected with pSuNeo-SVV.

Figure 3 SMMC-7721 cells transfected with pSuNeo-SVV (A) and pSuNeo (B).

Figure 4 Growth curve of SMMC-7721 cells.