Effects of unsaturated fatty acids on calcium-activated potassium current in gastric myocytes of guinea pigs

Figure 1 Effects of AA and LA on I_K(Ca). A: Raw traces of AA on I_K(Ca) at different concentrations; B: I/V relationship of AA on I_K(Ca). Peak values were normalized to the values obtained at 100mV under control condition (n = 8,^aP>0.05, ^cP<0.05, ^bP<0.01 vs control);C: Dose-dependent increase of AA on I_K(Ca) (n = 8, ^cP<0.05, ^bP<0.01 vs control); D: Increase of AA on STOCs; E: Raw traces of LA on I_K(Ca) at different concentrations; F: I/V relationship of LA on I_K(Ca) (n = 8, ^aP>0.05, ^cP<0.05, ^bP<0.01 vs control); G: Dose-dependent increase of LA on I_K(Ca) (n = 8, ^aP<0.05, ^bP<0.01 vs control).

Figure 2 Comparison of different unsaturated fatty acids on I_K(Ca). A: Raw traces of 10 μmol/L OA, LA and AA on I_K(Ca); B: Increased effect of different unsaturated fatty acids on I_K(Ca) (n = 8, ^aP<0.05, ^bP<0.01 vs control).

Figure 3 Effects of PKC inhibitor and oxygenase inhibitor on AA-induced increase of I_K(Ca). A, B, C and D: Effects of AA on I_K(Ca) after pretreatment with H-7, indomethacin, 17-octadecynoic acid and nordihydroguaiaretic acid, respectively(n = 8, ^aP>0.05, ^cP<0.05, ^bP<0.01 vs control); E: Comparison of AA on I_K(Ca) before and after pretreatment with H-7, indomethacin, 17-octadecynoic acid and nordihydroguaiaretic acid, respectively(n = 8, ^bP<0.01 vs control, ^aP>0.05 , ^cP<0.05 vs AA).